Sentence examples for passages split from inspiring English sources
M0202, HEM and NHEM melanocytes were passaged (split 1 3) routinely every 10 or 11 days, or harvested when they had reached confluency.
The culture was propagated for two passages and split 1 : 4 after achieving 75 80% confluence in order to achieve the cell number required for 3D cultures assessment.
For MEFs, 5×104 cells/well at passage 3 (split at 1∶4 at each passage, used prior to immortalization) were transfected with expression constructs driven by the CMV promoter, and done in triplicate.
The stabilized cells (3rd 20th passage) were split into microtitration plates to get a concentration of 100 000 cells/mL and the differentiation to macrophages was induced by a phorbol myristate acetate (PMA) as we described previously [40].
The mES cells were passaged and split (ratio 1 8) every 2 days.
OB1 cells, at the 15th passage, were split as previously described, and were transfected with siRNA-RB or with non-targeting control.
The two bridges that straddle the Bosphorus, the passage that splits Istanbul and separates Europe from Asia, are very much at the center of these problems: few other bits of tensile steel have so altered the destiny of a city.
To get through small passages, LocoRocos can split themselves into mini-LocoRocos, and when they re-form they make the sounds of children playing.
Cells were used for up to 20 passages and were split every 2 4 days.
When aggregates of polygonal and bizarre epithelioid cells appeared in the background of spindle-shaped cells in the fourth passage, the cells were split and thereafter passaged every week.
