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Then, every 5 days, the infected cell cultures were split; every 10 days (two passages), samples were lysed and frozen for colony-forming unit (cfu) analysis.
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The decrease in TRP-1 mRNA levels from the 4th passage to the 7th passage samples were statistically significant compared to the 1st passage samples.
Values are shown as relative expressions compared to the first passage samples.
Values are shown as relative expressions compared to the first passage samples (mean±SD from three independent experiments; *significant at p≤0.05, Univariate ANOVA).
At each passage samples were collected for RNA analysis by snap freezing in liquid nitrogen and storing at −80 °C and for differentiation studies.
All six of the original rabbit HEV infectious plasma samples and the second-passage samples GDC54-188) of rabbit HEV were generated from rabbits and titered by real-time PCR.
The experiments were performed at passages 8 or 14 (low passage samples) or 25 (high passage samples).
Early and high-passage samples were analyzed by karyotyping.
The miR trascriptome profiles from the two 6th passage samples differed from each other as much as from the 4th and 5th passage sample.
At the appearance of cytolysis at each passage samples were withdrawn and their genomes completely sequenced.
KFT and OEE provided continue SARS-CoV containing Vero cell passage samples.
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