Fortunately, the flat passages pass by quickly, and the tide rises again and again, buoyed by Mrs. Parish's voice and those of her friends, colleagues, family and protegees.
Colter et al. reported that single cell derived MSC clones could be expanded up to 50 population doublings in about 10 weeks if cultured by repeated passage at low density whereas cells stopped growing after 15 passages if passed at high cell density [31].
To test whether the expression score, as defined above, discriminates IFN/STAT1 pathway high (STATH) and low (STATL) expressors relative to B16F1 baseline, we performed QRT-PCR to profile eight cell lines from the P1 passage (B16F1 passed through the lungs of wild type C57BL/6 mice; Figure 1D and Figure S2).
Even with all of that marvelous whisky back home, the promise of America was a chance that few who could find the passage would pass up.
After I discovered them while in college, it became a rite of passage to pass along their absurd, surreal sketches to anyone who would listen.
The Florida strain has the longest passage history, being passed continuously since isolation, and the Puerto Rico strain has only been passaged once since coming to our laboratory.
Despite the decades that have passed, some passages were redacted because they contained still-classified information.
"Dark Voyage" is the eighth of his wonderful spy novels, and there are passages that could be passed off as the work of both a meticulous narrative craftsman at the top of his game and a symbolist poet.
Nasal and bronchial epithelial cells were initially expanded onto irradiated 3T3 fibroblasts (passage 0) and then further passed to grow into passage 1 (P1) which was later frozen in liquid nitrogen.
Among other things, the "may" passage is newer law, passed in 1989 compared to 1951, and it deals specifically with the question of deadlines.
