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OASFs were cultured with 1 μg/ml HMGB1 plus 10 ng/ml LPS for five passages (media were replaced every 3 days with fresh HMGB1 LPS complex) to get the RASF-like transformed OASFs (tOASFs).
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For passage, media was aspirated off and cells were washed with sterile PBS to remove any remaining serum.
Since SLI is undetectable in the passaging media it must, therefore, be synthesised by the cells.
For experiments determining the effect of oxygen tension on protein expression, MCF-7Ca and LTLTCa cells were plated in passage media and incubated either under normal (20% O2 at 37°C) or more physiological (5% O2; using a hypoxia chamber) cell culture conditions for 24 hours.
"Entrances and Passages: Mixed Media Textiles," an exhibition featuring textile and fiber arts by Barbara Schulman.
F3 cells were grown for two passages in media containing 250 μg/ml 6-thioguanine. 100 μg of linear targeting vector plasmid was introduced into 1.5 × 10 F3 ES cells by electroporation.
"Americans' awareness of key elements of the law has declined somewhat since passage, when media attention was at its height".
Before his passage into media don-dom, Schama was a distinguished historian of the French Revolution and the early modern Low Countries.
Mr Klitscho had negotiated their safe passage, local media reported.
Subsequently stable, variable expression level cell lines were passaged in media containing 40 50 μg/ml zeocin.
In the flank model of GBM using BT11, 20 nude mice (NU-FoxnNu) were injected with 1 × 10 primary human glioblastoma cells at the 6th passage in media:Matrigel (1 1).
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