Monitoring the expanded GAA repeats in FRDA iPSCs over several passages, we detected both expansions and contractions, whereas normal repeats in control iPSCs remained stable (Fig. 6A).
Both in early passage and senescent cells we detected 11q TERRA transcripts, however, we could not detect TERRA transcripts emanating from 7q in both cell passages (Fig. 4A).
In all patients we detected significant transperitoneal passage of PChE during peritoneal dialysis.
In both groups of examined patients we detected the significant passage of PChE from blood to dialysis fluid (P = 0.000089 for peritonitis-free patients and P = 0.002218 for patients with peritonitis).
When the vitamin E effect was analyzed, we detected downregulation of P21 gene expression (p = 0.015) in passage 9 HUVEC cultures but not in early passage cultures) but detected no effect regarding the P16 expression).
Similarly, we detected no CWD PrPRES (PrPCWD) in CWD-infected cells treated with PrP-RVG-9r siRNA after passage 12, while we detected PrPCWD in control-treated cells as far as passage 18 (figures 5C, D and F).
The proliferation rule of DMS/PCs at passage 3, passage 17, passage 31, and passage 45 was detected by cell hemocytometer, and the growth curve was drawn following the counting data (data not shown)).
As a fly walks back and forth from one side of the tube to the other end, its passage is detected and counted by an infra-red beam which bisects the tube, and the accumulated count totals are reported to the host computer at the conclusion of each reading period.
After second passage, PrPres was detected in a single mouse inoculated with one of the three H-BSE isolates.
Porcine DFAT cells at passage 4 (P4) were detected by flow cytometric for cell surface antigen.
