Sentence examples for passage was done from inspiring English sources

Cell passage was done every third day.

A blind passage was done with all cultures showing no CPE after 10 to 14 days.

Neurosphere passage was done every 7 10 days, and the cells used for experiments were harvested after passage 2 3.

The guinea pig passage was done by the inoculation of five animals, one for each strain, by the intraperitoneal route with 1.0 ml of a B. mallei suspension in PBS containing 10 UFC [ 23].

The third method for hEB passaging was done by draining away the medium to fasten the EBs to the dish, then the EBs were cut by a McIlwain tissue chopper or sterile razor blade.

Passaging was done using Trypsin (SIGMA).

An adequate review of the immunocytochemical analysis for NET markers especially in the low passages was done previously (Pfragner et al. 2009).

After 5 6 days, the cultured HDFs were harvested by trypsinization and culture expand into new T25 culture flasks (Nunc, Denmark) with expansion degree of 1 : 4. When the subcultures reached 80 90% confluence, serial passaging was done by trypsinization and the number of population doublings (PDs) was monitored until HDFs reached senescence.

Passages were done every doubling time i.e., 3-4 days.

Further passages were done with 1 4 cell dilutions.

Medium was replaced every 4 days and passages were done when the cells reached, 80% confluence.