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As any armchair anthropologist will tell you, young men have long engaged in tribal rites of passage to test courage and toughness.
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It includes longer passages to test reading comprehension and requires more essays, including one based on a spoken passage.
To monitor the stemness and differentiation potential of long-term cultured Sca-1+ cells, we selected the 7th, 28th, and 53th passages to test early, middle and late phases of culture.
The significant groups were further analyzed by crossing coating and passage factors to test for any specific differences observed between passages of different coating groups.
To test for the stability of these cells over several passages and to test their stability in different media, XEN cells were thawed and cultured as previously described.
A differentiation assay was performed on passage 8 MSCs to test their multi-lineage differentiation capacity.
Field Smith says: "They have to go through a rite of passage to enter this weekend, to test them as men.
Many spectators said they would wait for others to test the passage.
In order to test the passage stability, 106 transgenic P. berghei parasites were inoculated intraperitoneally (i.p).
FLS-RA cells were used to test from passage 3 to 8.
All HUVECs were analyzed prior to passage 8. Co-immunoprecipitation experiments to test for cis versus trans interactions between Nrp1 and β8 integrin were performed in HEK-293T cells.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com