hNSCs were derived from hESCs (H9, University of Wisconsin, Madison, WI, USA; and HSF-6, University of California San Francisco, San Francisco, CA, USA) and hiPSCs (IMR 90-4, usingrsity of Wisconsin) using an in vitro differentiation protocol that includes neural induction on the feeder layers of MS5 stromal cells, followed by consecutive cell passages to select NSCs, as previously described.
This indicates that OVCAR-3WT in vivo passage contributed to select clones with these cellular properties, which cannot be correlated with trastuzumab long-time exposure, but only to in vivo passage.
We used serial passage of cells to select for spontaneous changes that resulted in increased growth under fermentative conditions (microaerobic).
We then passage these viruses to select for functional variants, and use Illumina deep sequencing to determine the frequency of each HA mutation before and after this selection for viral growth.
Next, the neurospheres were passaged multiple times to select against lineage-committed progenitor cells not capable of self-renewal.
Actually, our selection regime with expanding host cell populations and serial passage transfer will tend to select for viral clones that are able to expand fast.
While every effort will be taken to select passages of a fairly consistent level of difficulty from one exam to the next and from one language to another, some variation will inevitably occur.
The cells were passaged once a week to select for tumorsphere culture cells.
This detailed analysis technique allows those responsible for student learning at any level to select a passage that could appropriately represent the type of text from which they expect their students to learn.
In order to transform EP156T cells to prostate cancer cells, the cells were kept growing at passage 43 at high confluence to select cells that survived contact inhibition.
