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The Frenchman duly arrived at the KC Stadium requiring safe passage to avoid the knives being further sharpened for him.
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The hMSCs used in this study were isolated from middle-aged donors; were short-term cultures and used for a limited number of passages to avoid the interference with senescence (Figure 1).
JKT-1 cells were cultured up to 38 passages to avoid the drift of these cells.
All cell lines were used within 10 to 12 passages to avoid variations.
Therefore, in all experiments, primary osteoblasts were used without additional passaging to avoid any potential phenotypic drift.
The results of this study show, that experiments, which examine gene expression of RASF and shall reflect or imitate an in vivo situation, should be limited to early culture passages to avoid cell culture effects.
Co-culture experiments were carried out with fibroblasts of passage 2 3 to avoid cell-aging effects.
Karyotype analysis was done on low passage cells to avoid artifacts that could arise from long term in vitro cultivation.
Strain passage was minimized to avoid plasmid deletions due to the recA + nature of JS006 and the high complexity of oscillator plasmids or triple-reporter plasmid.
For continuous expansion, half of the medium was replaced every day, and cultures were passaged for no more than 3 consecutive passages in order to avoid long-term culture-related effects.
Still, when the music turned impetuous, Mr. Pollini sounded almost impatient to get through these passages so as to avoid obvious dramatic effects.
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