Sentence examples for passage them from inspiring English sources

When the colonies get big enough and start to touch other colonies is time to passage them.

Thus, 3D alginate culture seemed to be superior for NC culture as a higher fraction of large NC were revealed after 34 days of culture, compared to the identical cell population cultured in 2D monolayer and with the requirement to passage them three times for the same number of culture days.

The Turkish decision to allow entry to the fleeing Syrian Kurds came a day after Ankara had refused them passage, blocking them at the border fence.

The cells could be maintained in an active state for more than two months without the need of passaging them.

When the cells attached, we passaged them upon confluence.

Cells were kept in logarithmic growth phase by routinely passaging them twice a week and were plated 24 h prior to treatments.

Therefore, we digested the spheroids using collagenase to obtain single cells and passaged them in the new NCP wells to eliminate debris.

Current protocols for obtaining parasite DNA for whole genome studies from P. falciparum field isolates consist of culture adapting the isolated parasites and passaging them for 3 4 weeks.

Stocks of WNVKUN MRM61C and WNVNY99 4132 that most closely resembled the low-passage level WNVNSW2011 were prepared by electroporation of BHK21 cells with WNVKUN or WNVNY99 RNA (prepared from corresponding infectious cDNA clones) and passaging them 1× in C6/36 cells.

We cultured primary HMEC from four histologically normal breast tissue specimens and grew them in serum-free MEGM medium under standard adherent conditions [ 5], subdividing and passaging them before they achieved confluence.

Replicative senescence was induced in HCAEC cells by passaging them every 2 days in MesoEndo Cell Growth Medium 212-500 (Cell Applications, Inc .. Oncogene-induced senescence was induced in ER Ras-IMR90 by trER Ras-IMR90 hydroxytamoxifen for 5 days.