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In addition, infectious dose experiments demonstrate that increased infectivity in mosquitoes was also not achieved through sequential passage (table 3).
Similar to growth kinetics results, mosquito-passaged SLEV (MP20A and MP20B) also showed no significant change in Cx. pipiens infectivity as a result of passage (table 3).
Infectious dose experiments confirmed that this difference could be attributed to a greater than 30-fold increase in infectivity resulting from chicken passage (table 2).
Results indicated no significant differences in infection rates at any dose (Chi-squared, p>0.05) and equivalent ID50 values for SLEV before and after chicken passage (table 3).
A much shorter incubation period (∼160 days), similar to that with secondary passed sCJD was observed upon early brain passage (Table 1), suggesting that distinct prions had propagated in early and late mice.
In contrast, neither complete or partial tumour regression nor any tumour stabilisation were observed beyond the third passage (Table 1 and Figure 1).
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In order to assess possible changes in virus infectivity resulting from passage, infection rate at various doses and ID50 of SLEV were determined in chickens or mosquitoes before and after passage (tables 2 and 3).
Moreover, unlike the traditional 2i/Lif condition which could only support NPG ESCs at early passages (Table S1), our new culture condition allows long-term maintenance of the self-renewal circuitry of NOD-scid Il2rg − / − ESCs with normal karyotypes and in vitro and in vivo pluripotency.
Samples selected from the training corpus showed that the following four classes of information request appeared to be common, and led us to produce sets of indicators for accepting or rejecting passages (Table 4); this offers the possibility to filter the chat logs to identify potential predators.
C2 ES cells were passaged in RESGRO™ medium for several passages (Table 1).
Thirteen out of fifteen (86.67%) genes examined remained differentially expressed over 13 passages (Table 2).
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