In his original draft of the Declaration of Independence, he included a passage, subsequently deleted by the Continental Congress, blaming both the slave trade and slavery itself on George III.
(Virginia's plantations were already well stocked with slaves, so ending the slave trade posed no economic threat and even enhanced the value of the existent slave population). In his original draft of the Declaration of Independence, he included a passage, subsequently deleted by the Continental Congress, blaming both the slave trade and slavery itself on George III.
Students tend to pick answers which sound the most complex or sophisticated and will sometimes favor one answer before they refer back to the passage, subsequently falling victim to confirmation bias when they see a part of the passage germane to the choice they selected arbitrarily.
It is particularly interesting because it shows passages subsequently excised in Congress, such as Jefferson's lengthy condemnation of slavery.
In order to analyze the surface expression of CD105 by Flow Cytometry, magnetic separation was carried on at passage 3. Subsequently, CD105+ signal was monitored at passages 3, 4 and 5. Cell surface antigen phenotyping of CB, AT and BM derived hMSC was performed at passage 3. CD105 expression level in CD105+ enriched CB-hMSC population was monitored at passages 3, 4 and 5.
The mutants that were differentially cleared by SP-A during the in vivo passaging were subsequently retested by single PCR of bacterial cells recovered from three separate mice as previously described [17].
Induced pluripotent stem cells (iPSCs) generated from adult fibroblasts of X-CGD mice were adapted to feeder-free condition for five passages and subsequently characterized for stem cell morphology (round shape, large nucleus, and scant cytoplasm), alkaline phosphatase activity, and expression of pluripotency markers Sox2, Oct4, Klf4, Nanog, SSEA-1, and c-Myc (Fig. 1A).
Cells were propagated for only one or two passages and subsequently were seeded on collagen-coated glass plates for imaging.
The cytarabine-resistant K562 cell line was generated by continuous exposure of K562 cells over several passages to subsequently increasing concentrations of cytarabine (0.5 to 256 ng/ml).
Several weeks prior to infection of 'Amakihi, an aliquot was thawed, deglycerolized and inoculated intravenously into a Pekin Duckling (passage 7) and subsequently passaged two more times in Pekin Ducklings (passages 8 and 9) to increase parasitemia prior to infection of mosquitoes.
To achieve combinations of retroviral infections, cells were infected with LXSN-16E6, LXSN-16E7, LXSN-16E6E7, LXSN-hBmi1, or LXSN alone, at passage 2, and subsequently reinfected with MSCV-hTERT at passage 3.
