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The Safe Passage Study was designed to investigate the role of PAE in a continuum of poor peri- and postnatal outcomes.
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The biologic clone of WNV used as the parental strain for passages studies was isolated from WNV NY003356 by three rounds of plaque purification on Vero cells (ATCC #CCL-81) and WNV CP40 was obtained by 40 sequential passages on C6/36 mosquito cells (ATCC #CRL-1660) as previously described [ 19].
The passage analyzed in our study was much later compared with the studies on BMMSCs or ADMSCs and also much later than the passages that were used in clinical applications.
The definition of "Diarrhea" in this study was the passage of at least three loose or watery stools in any 24-hour period.
The stock of PRRSV used in the study was the 8th passage of a Taiwan field isolate, PRRSV tw91, at a titer of 10 TCID50/ml, which was prepared and titrated on MARC-145 by cytopathic effect (CPE) [ 18].
While a first passage of an agent between species is not normally sufficient to confirm strain identity, the similarities identified at first passage in this study are striking.
Early passage HDF used in this study were at passage 15 and MTF at passage 3. HDF were cultured until proliferation ceased (monitored by daily microscopic observation) and incubated for 2 more weeks prior to harvest.
The MSCs used in this study were passage 4 cells.
Another inhibitor of NPC passage crucial for this study is the dominant-negative Importin β fragment (Kutay et al, 1997).
The cell lines used in this study were passaged for fewer than 6 months after resuscitation and the passage numbers were 5 20.
In order to reduce the noise generated in the environment during a train's passage, studies have been carried out to model the track behaviour in dynamics.
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