Sentence examples for passage stock from inspiring English sources

The C. burnetii strains used in the study were propagated in cell and cell-free cultures in triplicate from the low passage stock cultures stored at −80 °C.

Because protein expression may be reduced in very high passage insect cell lines, we used High Five cells from a relatively low passage stock (84 to 95 passages) for this study.

Low passage stocks of HSV-1 McKrae and KOS were obtained from master stocks prepared by David Bloom (UF, Gainesville, FL).

Fibroblasts were expanded and second passage stocks were frozen in liquid nitrogen.

To confirm this, and to determine if infectious virus had been produced in these transfections, second passage stocks of Δ47, ΔL4 and WT viruses that had been recovered from the genome transfections described above were used to infect cells that were then analysed for early and late viral antigens by immunofluorescence.

All cells were passaged for <3 months before renewal from frozen, early-passage stocks.

LNCaP cells have been passaged directly from original low-passage stocks (2009), and we confirmed PC-3 cell identity by STR profiling in 2010 (Cellbank).

All cells were cultured under a humidified atmosphere of 5% CO2 at 37°C in RPMI 1640 medium (Sigma, St Louis, MO, USA) supplemented with 10% fetal bovine serum and were passaged for ⩽3 months before renewal from frozen, early-passage stocks obtained from the indicated sources.

Finally, Vero cells seeded in 2 flasks of 75 cm were injected with 1.5 mL of the second passage virus stock in a total volume of 25 mL to generate a third-passage viral stock.

Bulk preparations of MVA.HAV were made from low passage MVA.HAV stock grown for 3 days in CEF cultures (SPF grade, IAH Compton, UK) as previously described [69].

The resulting population was titrated and used as a passage 0 stock.