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Values are shown as relative expressions compared to the 2nd passage sample.
The miR trascriptome profiles from the two 6th passage samples differed from each other as much as from the 4th and 5th passage sample.
In analyzing this batch of samples, we assessed all the positions across the viral genome in which there was a significant variant in the alternating passage sample that had cycled through mosquito cells, which was not found in any of the other samples (that encountered only mammalian cells) above a rate of 0.001.
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The decrease in TRP-1 mRNA levels from the 4th passage to the 7th passage samples were statistically significant compared to the 1st passage samples.
Values are shown as relative expressions compared to the first passage samples.
Values are shown as relative expressions compared to the first passage samples (mean±SD from three independent experiments; *significant at p≤0.05, Univariate ANOVA).
At each passage samples were collected for RNA analysis by snap freezing in liquid nitrogen and storing at −80 °C and for differentiation studies.
The experiments were performed at passages 8 or 14 (low passage samples) or 25 (high passage samples).
At the appearance of cytolysis at each passage samples were withdrawn and their genomes completely sequenced.
KFT and OEE provided continue SARS-CoV containing Vero cell passage samples.
Identical passage samples were derived from the same freezing stock and have been sequenced by Complete Genomics and Illumina platforms.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com