If the caves were formed by pressure flow beneath the water table, the passage profile can be irregular, with both downsloping and upsloping segments.
In the course of serial passages, RNA profiles showed a progressive increase in rearranged segment 7R intensity, along with a decrease in wt segment 7 intensity, which actually became undetectable at passage 9, indicating that segment 7R had replaced its wt counterpart in the viral progeny.
Cells were used experimentally up to passage 3. Profiling was performed using six paired samples of normoxic vs. hypoxic (24 h, 2% O2) osteoclasts.
Viral progenies resulting from mixed infections were serially propagated in MA-104 cells and at each passage the RNA profiles of the viral populations were analyzed by PAGE.
Early and late passage had identical profiles and did not match with any other cell line in the DSZM database.
We have previously observed isolates that on second passage, produced similar profiles to UK-2, which on third serial passage, presented a LP compatible with 87V (unpub. obs).
To determine if similar epigenetic modifications are induced by long-term culture of fibroblasts, we have compared DNA methylation profiles of passage 3 and passage 21 of fibroblasts (6 year old donor, othoplastic).
Here, for example, is a typical passage from his profile of Louis XVI: "His early childhood was nothing short of desolate.
In one passage from his Profile of the Chief Justice, Jeffrey Toobin wrote, On issues of Presidential power, Roberts has been to Scalia's right — a position that's in keeping with his roots in the Reagan Administration.
The same is true of the recipes in Beaneaters & Bread Soup: Portraits and Recipes from Tuscany (Quadrille £20, pp224), by photographer Jason Lowe and his American wife, Lori De Mori - and of the prose passages, which are profiles, for the most part, of their neighbours in the Tuscan hills.
