Sentence examples for passage population from inspiring English sources
Gene expression profiling was performed between NHEK infected with retroviral vector expressing GRHL2 (NHEK/GRHL2) and the empty vector (NHEK/LXSN) at early passage (population doubling (PD) 14) or late passage (PDs 23 and 30).
Early passage (population doubling < 35) IMR-90 human lung fibroblasts (Coriell repository, NJ) were sub-cultured in Dulbecco's modified Eagle's medium (Gibco) and Ham's F10 medium in a 1 1 mixture plus 15% fetal bovine serum.
Parental MCF-10A cells were obtained from the stock collection at the Garvan Institute of Medical Research, Sydney, and represent an early passage population from an import from ATCC.
Early passage (population doubling < 35) IMR-90 human lung fibroblasts (Coriell Cell Repository, Camden, NJ) were sub-cultured in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA) and Ham's F10 medium in a 1 1 mixture plus 15% fetal bovine serum.
When two different batches of FBS and HS were compared on maximum passage, population doubling time and total protein content of EBF, there was no remarkable differences between them.
This is in contrast to a gradual decrease over time in WT BJ cells in which we observed an ultimate 10-fold down-regulation at senescence compared to expression during early passage populations.
We compared 'young', proliferating (P), early-passage (population doubling, pdl ~20) WI-38 fibroblasts with late-passage, senescent (S, pdl ~54) WI-38 fibroblasts.
It is yet unclear how many passages, population doublings or senescence-associated molecular changes are acceptable to grant optimal therapeutic effect for the different applications.
These studies, which also show no difference in the number of mutations fixed in alternate and sequentially passaged populations, do not necessarily support the idea of a dampened rate of genetic change as a consequence of host cycling.
Flow cytometry data showed positive expression of CD90, CD73 and CD105 in all third-passage populations (94.36 ± 6% of events acquired).
Haematopoietic lineage markers CD34, CD45, CD19 and HLA-DR turned out to be negative throughout all third-passage populations, verifying the accepted criteria for the MSCs defined by the ISCT [ 11].
