Those surviving the LD90 dose were passaged over several weeks in Tf Hp-free medium and Tf Hp-freeted for their dose–response to Tf–Hp (second treatmedium
To address the question whether CBS directly controls entry of human endothelial cells in cellular senescence, the protein was expressed from a lentiviral vector in early passage HUVEC, which were then monitored over several passages.
To evaluate the critical effects of this passaging procedure in cell culture on gene expression, RASF were therefore cultured over several passages followed by cDNA array analysis for up to eight passages and comparison of early passages to higher culture passages was performed.
To evaluate whether cell culture effects take place in RASF cultures over several passages, we performed cDNA array analysis for up to nine passages.
To test, whether sphere cells could be serially enriched, we seeded 20000 sphere cells over several passages into the SC-medium and determined the number of spheres at each passage (Figure 1B).
For 'TET-OFF' experiments, ESCs were cultured over several passages (weeks) on feeder cells in the absence of DOX and were subsequently passaged onto feeder-free plates prior to the inhibition of HA-H3.3 expression.
PEV PCR is more rapid and less laborious than the time-consuming virus isolation by tissue culture techniques over several passages and serotyping.
It is noteworthy that lentiviral transduction shows the expression of cytosolic fluorescent reporter proteins to be stable in human stem cells and differentiated neurons28: this is in line with robust EYFP expression in iNGN cells over several passages.
In the case to be heard Tuesday in Milan, Fedele Confalonieri, the chairman of Mediaset, is suing over several passages in Mr. Stille's 2006 book on the rise of Mr. Berlusconi, "The Sack of Rome".
Continued culture in this manner resulted in the progressive loss of mCherry expression over several passages (data not shown).
