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The characteristic cytopathic effects (CPE) of clumping and fusion of Vero cells were observed starting from the 7th passage onwards.
Our studies indicate that replicative senescence of MSC preparations is a continuous process starting from the first passage onwards.
Adherent cells from the first passage onwards were termed TMSCs.
From the 40th culture passage onwards, the PA 16/23 cells ceased to grow, either spontaneously or in response to exogenous stimulants.
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Taken with the data discussed earlier, Pif1 contributes to good growth of tlc1Δ mutants from passage 2 onwards, yet inhibits growth of cdc13-1 mutants.
Starting from the third passage and onwards, on average approximately 99% of the SF cells were fibroblasts, with very few (< 1%) contaminating macrophages, T cells and B-cells detected by fluorescence-activated cell sorting analysis.
At this point in spite of upregulation of MDR1, a multidrug-resistant profile was not evident in the MCF-7TaxR cells from passage 23 onwards as only a modest increase in doxorubicin resistance was apparent and resistance to other MDR-associated agents, including etoposide was not seen.
They came because they thought they could find onwards passage to Australia.
Confirmation of more than 90% purity of SF cell populations at passages three and onwards involved staining for prolyl 4 hydroxylase (5B5 antibody, Abcam, Cambridge, MA, USA) and fluorescence-activated cell sorting analysis.
@candicepires urged me onwards, to the Passage Cafe, a cracking little dim-lit French bistro, where I raced though my main before gorging on chocolate soup and black pepper ice-cream.
Historically, Democrats were the voice of the working classes; but already from 1964 onwards (with the passage of the Civil Rights Act), southern Democrats turned increasingly to the Republican party.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com