Studying differentiation, we cultured tissue explants and cells from passage one on hanging membranes either with access to medium from both basolateral and apical side or in an air-liquid interface with access to medium only from the basolateral side.
Primary cervical cells and cells of passage one were cultured on Millicell inserts either with access to growth medium from both basolateral and apical or in air-liquid interface with access to growth medium only from basolateral.
For primary antibodies and protocol details see Table 2. Either tissue explants or 1 × 105 cervical cells in passage one were grown on Superfrost Plus® slides (Gerhard Menzel, Glasbearbeitungswerk GmbH & Co. KG, Braunschweig, Germany) until confluence.
The media was changed every 2 days, and on reaching confluence (within 2 weeks) the cells were passaged (passage one) into a 225 cm2 tissue culture flask.
By Shon Arieh-Lerer June 26 , 2017Passage One Hi, sweetie!
Rough passage One to watch Queen of Madison Avenue ReprintsSome branded manufacturers have set up their own in-house divisions to market authorised generics.
They suggested they had lined up the nine votes needed for passage, one from Britain and the rest from nonpermanent members.
On the jacket the girl exuberantly flings out her arms and legs in the passage (one might say the rift) between two worlds, city and country.
