Passage of tumor tissue was conducted by mincing the tumor tissue, passing it through a sterile tissue strainer, washing it in PBS, and re-injecting 50 mg of tumor in 200 µl of 80% Matrigel.
Surprisingly, a second cell type, present only in early passages of tissue-derived cultures, showed extension of life span and infrequent immortalization by E7 alone.
Furthermore, the low sensitivity of microscopy, the low volume of brain examined under the microscope (30 μl), the absence of concentration method, and not performing further passaging of tissues of the mice without detectable cysts could have contributed for the absence of cysts from seropositive mice.
The mechanism of cyst formation is similar to that of paralabral cysts in the shoulder, with the passage of synovial tissue and/or fluid to the adjacent soft tissues through a labral tear, the majority occurring in the anterosuperior part of the acetabular labrum [17, 19].
Seq1: Contains 13 frames from colon with perspective passage motion of tissues.
Single-cell suspensions of the spleen were obtained by mincing tissue with glass slides and subsequent passage and scraping of tissue in a ThermoShandon biopsy bag (Thermo Fisher Scientific).
In epithelial cells, the ECM combines the use of a highly organized collagen network, hyaluronic acid, and a number of proteins to prohibit the passage of macromolecules through tissue.
It remains to be clarified if the inability to detect a NIRS signal was due to some muscle characteristic that also interfered with contractions or with the passage of current through tissue.
Transmission of the kuru syndrome from chimpanzee to chimpanzee has now been effected, with a shortening of the incubation period to 1 year from the 1 1/2 to 2 1/2 years found on intracerebral passage of human brain tissue to chimpanzee.
DOI: http://dx.doi.org/10.7554/eLife.03679.012 10.7554/eLiFigure79.013 Figure 5 figure supplement 2. Infectious virus rebound after blind passage of faecal and tissue sample homogenates in RAW264.7 cells.
