Sentence examples for passage of strain from inspiring English sources

Genetic alterations were similarly acquired in the 3'-repetitive region of futB in three out of the thirty isolates that were recovered after in vivo passage of strain 67:21 in mice.

Nevertheless, major changes in the O-antigen chain were observed after passage of strain 67:21 in vitro with a small bottleneck, as well as in vivo in mice.

The virus used to infect animals in these studies was a second Vero cell passage of strain NY385-99, orisolated isolated from the liver of a Snowy Owl (Nyctea scandiaca) that died at the Bronx Zoo during the 1999 WNV epizootic in New York City (7).

The strains Rlow and Rhigh derive from different passages of strain R grown in artificial medium [ 14, 15].

The 7th passage of PRRSV strain Ter-Huurne, a EU-strain of PRRSV, propagated on lung macrophages was used.

The REV-T strain was also cultivated in CEKs and harvested 5 days post inoculation Third passage of each strain in CEKs was suspended in preservative medium containing 10% DMSO, gradually chilled and finally frozen in liquid nitrogen.

Continuous passage of this strain for 100 inoculation times demonstrated that a higher percentage (>95%) of cells maintained the plasmid when cultivated under selective pressure (ampicillin) than under nonselective conditions, suggesting that the presence of antibiotics in the medium prevents the loss of the pcDNA-CCOL2A1 plasmid.

This property may reflect the past history of passage of this strain that was artificially passaged in eggs and then subsequently in the brains of mice.

It is intriguing that in spite of the long passage of this strain in the community there has been no further development of drug resistance.

Following the first passage of this strain, EDTA blood was harvested at peak bacteremia and stored in 1.0 mL aliquots at −80°C.

These data suggest that this recent clinical isolate was not attenuated in its ability to multiply in mice and that intravenous passage of the strain through mice did not result in an enhanced ability to multiply in BALB/c mouse lungs.