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The time for the passage of cells through the epidermis, from formation to desquamation, has been variously estimated at one to three months.
Nitric oxide (NO) derived from iNOS then prevents a break-down of the BBB and non-regulated passage of cells.
The G1 cell cycle checkpoint controls passage of cells from the first gap (G) phase into the DNA synthesis (S) phase.
In this work, we describe that the subcellular localization of NFAT5 and its capacity to bind to chromatin are sensitive to the passage of cells through mitosis.
0.05% Trypsin-EDTA (Invitrogen) was used for the passage of cells grown in FBS-DMEM and Accutase (Millipore SF006) for ES cells grown in KnockOut™ Serum Replacement (SR).
The retinoblastoma protein (RB) negatively regulates the passage of cells from G1 to S phase primarily by sequestering E2F transcription factors and chromatin modifiers critical for the G1/S transition.
Similar(18)
However, the capability of these stem cells for chondrogenic differentiation becomes gradually decreased with the passage of cell culture.
In this system, there is no passaging of cells, and therefore genetic diversity is lost only through negative selection, without the experimentally-imposed bottlenecking common in other platforms.
Cell passage stability was evaluated with 2 independent runs; each run included 2 passages of cells with the same NC and PC preparations on the same plate, 1 passage ≤ 10 (passages 9 and 10) and the other ≥ 20 (passages 21 and 22).
Differentiations were repeated with three successive passages of cells for a total of three grows.
Experiments were repeated at least 2-3 times using different passages of cells on different days.
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