Sentence examples for passage of ADSCs from inspiring English sources

The third passage of ADSCs was adopted for immunophenotype analysis.

The phenotype of ADSCs (passage 3) was evaluated by flow cytometry analysis (FACS, BD Biosciences, San Jose, CA) by using PE-labeled anti-human CD29, FITC-labeled anti-human CD34, FITC-labeled anti-human CD71, and PE-labeled anti-human CD90 monoclonal antibodies (Chemicon, USA).

However, a substantial alteration in the differentiation potential of ADSCs from both early and late passages (3rd and 14th, respectively) was found when the cells were switched to osteogenic differentiation conditions.

The phase contrast image of ADSCs on the electrode showed that the cells at passage 9 had a small and spindle-shaped morphology while the cells at passage 31 were flattened and larger than younger cells.

Figure 1 Gene expression analysis during chondrogenesis of ADSCs.

The value increased gradually during chondrogenic differentiation of ADSCs.

In order to meet clinically urgent demand of ADSCs, the cell cryopreservation was applied to the ADSCs long-term preservation.

Therefore, integrin β1 may be responsible for the maturation and degeneration of chondrogenic differentiation of ADSCs.

Cumulative population doubling time (CPDT) curves showed insignificant decline in the symmetric cell growth studied for up to 13th passages accompanied with small changes in the overall cell morphology and moderately declined fibronectin (FN) matrix deposition probably as a functional consequence of ADSCs ageing.

BMP-12 activated tenogenesis of ADSCs based on gene expression analyses.

Isolation and identification of ADSCs was performed as described previously[18] with modifications.