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These beneficial effects were most obvious at higher passage numbers.
Stability was tested for cell passage numbers and standard parameters using PCs.
All experiments were performed with cells at passage numbers between 5 and 25.
Fig. 1 Phase-contrast photo micrographs of cultures showing MSC lines at various passage numbers.
However, similar blastocyst rates were achieved when using higher passage (numbers 9 12) EG cells as donor cells.
By using lower passage (numbers 3 5) EG cells as donor cells, the SCNT efficiency was significantly lower compared with use of fetal fibroblast donor cells.
Cells were used at passage numbers less than 30.
Endothelial cell passage numbers did not exceed 25.
BliPSC lines were used within 12 to 30 passage numbers.
TiPSC lines were used within 10 to 30 passage numbers.
KiPSC lines were used within 20 to 30 passage numbers.
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