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Passage numbering of cloned cells started again with passage one in 96-well plates.
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These beneficial effects were most obvious at higher passage numbers.
Stability was tested for cell passage numbers and standard parameters using PCs.
All experiments were performed with cells at passage numbers between 5 and 25.
Fig. 1 Phase-contrast photo micrographs of cultures showing MSC lines at various passage numbers.
In vitro migration assays indicated that MSCs at passage number 3 have the highest migrating ability.
Independent variables were the passage number of the cells and the plasmid loading.
Fibroblasts were used till passage number 20 and UT-SCC cell lines till passage number 55.
NPCs at passage number ≤15 were used in all experiments.
Cells were used at passage numbers less than 30.
Endothelial cell passage numbers did not exceed 25.
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