First-passage negative pools were passaged a second time to confirm absence of avian influenza.
This passage of negative dialectics is central not only to this book, but is a crystallisation of Ford's artistic mode throughout the Frank Bascombe series – throughout, indeed, all his work, from the superb story collection Rock Springs to his previous, non-Bascombe novel, the masterly Canada.
Regardless of lamin A depletion, the formed iPS cells at early passage were negative for lamin A by immunostaining but positive for pluripotent markers, Oct4, Nanog and SSEA1.
Many deleted passages included negative findings about continuing problems in the department's hiring, retention and treatment of minority employees.
Effects of gut-passage were negative at the seed germination stage, but seedlings from gut-passed seeds grew taller, had more leaves, and suffered less damage from natural enemies than any of the other seedlings.
NCI-Meso17 which did not express mesothelin in early passages remained mesothelin negative in late passages.
Thus, Cioculescu argued, the character Zinca Mamonoaia is the writer's step aunt Catinca Momuloaia, while an entire passage sheds a negative light on Ion Luca (the unnamed "leading writer of the nation" who prostitutes his trade).
Cells beyond the third passage were >99% CD45 negative, and used between passages 4 and 9. Cultured RA FLS were treated with IL-17A or IL-17F 50 ng/ml (R&D Systems, Minneapolis, MN), IL-1β 0.1 ng/ml (Sigma-Aldrich), LPS 100 ng/ml (Sigma-Aldrich), TNFα 1 ng/ml (Biosource, Carmarillo, CA).
Samples showing no CPE after the third passage were considered negative.
The samples that did not show hemagglutination activity after a second egg passage were considered negative for virus.
After passage, DPCs were negative for Sox10 (Supplementary Figure 2(a)), but were still positive for P75 (Supplementary Figure 2(a)) and Nestin (Supplementary Figure 2(b)).
