Therefore, we performed several passages of the viral progenies to new turnip plants using sap-inoculation and, after each passage, monitored virus latency periods (Fig. 2).
The pre-passage monitoring extended to Twitter.
In addition to his work in promoting the Nares Arctic expedition, Markham followed the work of other Arctic explorers, organising a reception in 1880 for the Swedish explorer Adolf Erik Nordenskiöld after the latter's successful navigation of the North-East Passage, and monitoring the progress of the American expeditions of Adolphus Greely and George W. DeLong.
b HRP was added to the top chamber, and HRP passage was monitored over time.
In these same animals, a thermocouple wire (30 gauge Type K; Thermo Electric Co., Inc.; Brampton, ON, Canada) was placed just inside the nasal passage to monitor respiration rate [62].
In a typical experiment, of 629 total colonies obtained prior to the first passage, we monitored 24 that were clonally derived from single cells and, of these, 12 were frozen as before passage while 4 were propagated as distinct lines.
Firstly, the cells under investigation were selected from the same clonal population following a process of repeated passaging and monitoring of growth rate in order to minimise non-growth related variation.
Experimentally, a field-scaled three-dimensional (3D) experimental setup is developed to perform erosion experiments, while the erosion rate of the internal flow passage is continuously monitored and measured.
But local advocates have sought the passage there for years, monitoring the steady stream of turtle deaths, building the temporary fence that protects turtles there now and mounting a large grass-roots campaign that eventually won state support for a more permanent "eco-passage".
Planning, training, and education should allow a seamless move into the actual passage of legislation or monitoring of implementation efforts.
The field would also benefit from development of new experimental techniques for monitoring the passage of ligands through the protein pathways.
