Exact(3)
Whole blood was processed as described in passage methodology and viral titers were used to generate viremia curves.
Results demonstrate that the capacity for genetic change is large for SLEV and that the size of the mutant spectrum is host-dependent using our passage methodology.
It is certainly possible that optimization for infection, replication, and egress from midgut tissue is much different than optimization for infection and replication in exterior tissues, yet achieving adequately high titers for mosquito infection via bloodfeeding in the absence of intermediate amplification is problematic and, therefore, this passage methodology is not feasible.
Similar(56)
Not only did that planning methodology lead to the most successful legislative agenda in US history (a 96% passage rate), but the policies themselves also worked astonishingly well in achieving their objectives.
Thus passaging frequency and methodology are expected to affect hESC culture properties in a manner that would impact spin-EB formation, likely a relevant factor in previously published spin-EB protocols [34], [36].
Therefore, an analytical reliability-based methodology using first passage probability theory for failure assessment of corrosion affected oil and gas pipelines is presented in this paper.
As explained in the Simulation Methodology section, we calculate the mean first-passage time for atoms to reach the surface of the NP.
Using well-established methodologies, endothelial permeability was measured by the passage of FITC-conjugated 40 kDa dextran through a monolayer of human umbilical vein endothelial cells (HUVEC -expressing vGPCR [ 5, 13].
We sequenced the complete genomes of 908 laboratxory confirmed influenza B virus MDCK or MDCK-SIAT cell propagated isolates passaged 1 4 times from eastern Australia and New Zealand using a novel methodology (Zhou et al., 2014).
Because this strategy to delete genes requires passage of the parasites through mosquitoes it will be extremely difficult to adapt this methodology to P. falciparum.
19, 20 These assays, however, present strong ethical, logistical, and biological disadvantages, requiring high laboratory animal usage and several months for passage, with the significant risk of genetic bottlenecks, thereby confounding results obtained from such methodologies.
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