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An effective method, called the passage method, is proposed in this paper to alleviate the disconnectivity, short-circuit, and roughness problems ordinarily encountered by the existing methods.
In order to achieve the reliability analysis of motion mechanism under the above three hybrid uncertainties, this paper presents a method of combining the Poisson's assumption-based first passage method and a double loop iterative algorithm.
rES cell lines were stably maintained by the bulk passage method [12] using both collagenase treatment and disaggregation of colonies by pipetting using Pipetman P-1000.
Four strains were isolated by the passage method (Table A1).
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Due to different passage methods and culture conditions, the BCG vaccine used in different countries varied.
Weak-field radiofrequency transitions using the adiabatic-passage method play an important role e.g. in atomic-beam polarized atom and ion sources.
Only a small group of highly skilled virologists could make use of the mutations, he says, and the well-known passaging method is "not an 'Aha' idea".
Manual passaging method was applied until a stable cell line was established (normally between p4 to p8), and thereafter enzymatic transfers were done either by using Trypsin/EDTA or collagenase type IV [2].
We are now undertaking a more extensive strain comparison by our standard serial-passage method using inocula derived from this primary transmission experiment (Ritchie et al., 2009).
We use the passaging method outlined in Sect.
The hPSC-derived BMEC purification process was also optimized by exploring the passaging method and subculture seeding density.
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