Sentence examples for passage medium from inspiring English sources

BIO was removed from the secondary passage medium in order to eliminate the confounding effect of BIO on cell proliferation.

Noting differences between in vitro and in vivo data, in general, optimal expression patterns concerning chondrogenic differentiation were achieved by few passages, medium cellular yield, short membrane-holding time.

With the presence of BIO in the secondary passaging medium, a greater number of larger spheres developed.

Human ES cells were differentiated to EC by modifying an established neural rosette protocol (Wilson and Stice, 2006). 2 days before passaging hES cells, medium was changed to N2 medium (DMEM/F12 with 1× N2). 1 day before passaging, medium was changed to N2 medium supplemented with 1 μM of Dorsomorphin (DSM) (cat# 171261; Calbiochem/EMD Millipore, Billerica, MA, United States).

After the removal of pAUR112 from cells by repeated passage in medium containing only HYG (YPD + HYG medium), a-type derivative cells expressing the kanMX4 gene were selected on YPD + G418 plates.

These peaks occurred even in the absence of passage or medium change, which causes depletion of essential nutrients and acidity.

Further experiments using a 5-factor/2-level factorial design including Caco-2 growth conditions such as cell passage, culture medium composition, cell seeding time and density, and stimulation time were also performed.

Near the 20th passage, the medium was changed from Opti-MEM I to DMEM supplemented with 10% FBS and antibiotics.