Sentence examples for passage lines from inspiring English sources

Wt and mt viruses were used to infect MT4 and C8166 T-cells to establish 24 serial passage lines (six replicates for each virus and cell line combination).

Since the wt and mt virus have not been specifically adapted to either cell line we expected to observe a fitness increase for all serial passage lines [34].

Two HIV-1 strains were each cultured in two different T-cell lines (MT4 and C8166) for 110 days (with six replicas for each treatment; making a total of 24 serial passage lines).

Sequence analysis of the LTR region showed that inactivation of the NF-κB site in the mt virus was stably present up to the end of the experiment in all serial passage lines.

Each of the 24 serial passage lines were analyzed by sequencing ten clones for each region and this was repeated for four time points, whereas for a fifth time point (at 61 days) only Env was sequenced.

The most important finding in our study is that none of the twelve serial passage lines that were cultured on C8166 cells resulted in an increase of viral fitness.

Each late passage line or karyotypically abnormal subline (BG01V, SA02) is identical to the early passage line, showing no evidence of overgrowth by contaminating cells.

Four clones derived at late passage (40) resembled the late passage line.

Animals were trained to run along a narrow passage lined with a strip of white paper before carrying out the test run.

Additionally, STR profiling does not reveal karyotypic changes that occur in some high-passage lines and may have functional consequences.

Challenge inocula in the "immunization" experiments were 1-9 cellsgenic cells from 4 different passaged lines and in the whole-body radiation experiments, 1-10(3) cellsgenic cells derived from 11 different primary hosts and 4 different passaged lines.