Sentence examples for passage line from inspiring English sources

PCR fragments were subsequently cloned into a pCRII-TOPO vector (Invitrogen) and ten clones were sequenced (forward and reverse) from each region and serial passage line and for each time-point (at time point 61 only Env was sequenced) with the Big Dye Terminator cycle sequencing kit (ABI).

Nucleotide diversity and the transition/transversion ratio (R) were calculated with MEGAv3.1 [81] and the Kimura 2-parameter model was used to calculate genetic variation in each genetic region for each serial passage line (only time points were included when at least eight clones from each region were successfully sequenced).

Each late passage line or karyotypically abnormal subline (BG01V, SA02) is identical to the early passage line, showing no evidence of overgrowth by contaminating cells.

Four clones derived at late passage (40) resembled the late passage line.

The NO7-152 celineise is an in-house cell line and is an early passage line grown in the traditional manner using DMEM high glucose, 4% non-essential amino acids (NEAA), 20% FCS and 2 mM GlutaMAX (all from Gibco, Carlsbad, CA, USA).

Wt and mt viruses were used to infect MT4 and C8166 T-cells to establish 24 serial passage lines (six replicates for each virus and cell line combination).

Since the wt and mt virus have not been specifically adapted to either cell line we expected to observe a fitness increase for all serial passage lines [34].

Two HIV-1 strains were each cultured in two different T-cell lines (MT4 and C8166) for 110 days (with six replicas for each treatment; making a total of 24 serial passage lines).

As a cross-check, we also determined fitness of the C8166 serial passage lines on the MT4 T-cell line, which demonstrates that these viruses had not changed their fitness on MT4 cells (Table 3, ANOVA, F1,3 = 0.117-2.842, p>0.05).

Each of the 24 serial passage lines were analyzed by sequencing ten clones for each region and this was repeated for four time points, whereas for a fifth time point (at 61 days) only Env was sequenced.

The most important finding in our study is that none of the twelve serial passage lines that were cultured on C8166 cells resulted in an increase of viral fitness.