In addition, en passage labeled fibers could often be seen running dorsally through the surpracallosal subiculum and adjacent dorsal area 29a c (Figs. 7C,D,E and Fig. 8).
The late-life crisis brought on by semiretirement is related in italicized interstitial passages labeled "In Transition".
At 24 h after injection, 150 μL suspensions of hMSG-EpiPCs at passage 5 labeled with CM-Dil were injected via the caudal vein approximately including 2 × 106 cells).
Each word deleted from the passage was labelled once by its traditional, or dictionary, classification and then again by a more modern grammar descriptor.
Roughly, when [1,2-C2]-glucose is metabolized, anaerobic glycolysis produces mainly m2 lactate, whereas passage of labeled glucose through the TCA cycle (including anaplerotic pyruvate carboxylation, pyr→oaa) or the oxidative and non oxidative branches of the pentose phosphate pathway results in m1 and m3 lactate.
For flow cytometry, cultured cells at high passages were labeled with the following commercial antibodies: anti-CD140b-PE anti-CD13-PECY7 anti-CD11b-PE, anti-CD14-FITC, and CD45-APC (all from BD).
For intravenous (IV) injection via tail vein, atrium FACS sorted cells that have been cultured for 5 passages were labeled with PKH26 (Sigma) which is a lipophilic marker inserted into the membrane of viable cells according to manufacturer's protocol prior to injection for better cell tracking [ 27].
Segments of text were coded by labeling passages with concepts abstracted from this text.
To test the proliferation of transplanted hMSG-EpiPCs in vivo, a mouse model of two-thirds partial hepatectomy was established as previously described, after which hMSG-EpiPCs at passage 5 were labelled with CM-Dil and injected immediately, as described above.
However, interesting information regarding this point can be found in the work of Ross and collaborators: "RNA samples from two cell lines (MCF7 breast and K562 leukaemia) were collected on three different occasions (at different passage numbers), then labelled, hybridized and scanned independently.
