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The channel passage is shown in blue dots along a blue axis.
The measured kinetics of cell detachment from the plate surface with respect to trypsin incubation time after a single passage is shown in Fig. 3(a).
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Typical nuclei from each passage are shown in Figure 1D.
Representative images of sphere formation taken at every passage are shown in Figure 3D.
PCR products from transfected BHK-21 cells at the thirtieth passage was shown and the sequencing results were correct.
Representative images of flow cytometry-based ALDH activity analysed at every passage are shown in Figure 3B.
CometScore analyses from our two groups of cells (early and late passage) are shown in the box-whisker charts in Figure 7C.
The results of the IFA (the thirtieth passage) were shown in Figure 3, and the results of the second, fifth, tenth, and twentieth passages were well accordant with that of the thirtieth passage (data not shown).
The amplification plots of the real-time Q-RT-PCR at thirtieth passage were shown in Figure 5. Cells transfected with p3D1shRNA and p3D4shRNA showed an obviously lower level in RNA replication than the others groups.
One strain had no mutations in the promoter or attenuator and normal amounts of Omp C and Omp F. aOnly those mutations that arose following serial passage are shown.
The low-passage, hemadsorption-positive and virulent strain Rlow (10th passage) was shown to be cell-invasive in vitro and in vivo, whereas the high-passage, hemadsorption-negative strain Rhigh (164th passage) displays a highly reduced virulence and exhibits only marginal cell invasiveness [ 10- 16, 16].
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