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This is consistent with our previous study where we showed that additional in vivo serial passage is required to attenuate T2Bo than L17 and more severe clinical pathologies are associated with animals infected with T2Bo_V than those infected with L17_V, all suggest that T2Bo may be more virulent than L17 [ 14].
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In terms of these facts, larger number of MSCs in early passages is required from a single source and single donor.
We found that two in vivo passages are required to restore the induction of cell-mediated immunity to passenger antigens and maximum virulence to these strains.
ORF75c+ viruses all spread rapidly, whereas several cell passages were required before ORF75c− viral replication outstripped cell division.
We have previously demonstrated that two passages were required to isolate a homogeneous population of MSC using the plastic adhesion method [ 17].
Additional experience, gained with both research and passage of time, is required to further unravel the intricate relationship between LVADs and the kidney.
However, because of the large conformational changes that accompany DNA strand passage, the cofactor is required to promote overall catalytic activity.
The apex seals come in contact with one another at certain points and a smooth passage past each other is required.
Successful passage of this exam is required for students to take Calculus II.
Pneumothorax is common in lung biopsies, particularly when passage of aerated lung is required to reach a lesion which was the case in all three cases in our series that required therapy of pneumothoraces.
Prediction of particle passage times in the system is required for ensuring sterility and optimising product quality.
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