SIVagm strains, which are derived from African green monkeys (AGMs) [ 8], are used for infecting pigtailed macaques (PTMs), in which upon passage induces a progressive infection [ 13, 14], and rhesus macaques, in which SIVagm is functionally cured [ 15].
From this correlation, we suggest that a sequence of changes in miRNA expression occurred during the tissue-culture passage-induced neoplastic transformation of 10-87 VERO cells.
The process causes the time passage variations induced by the torsional vibration to average to zero, ultimately yielding the time stamps caused by the passage of each respective encoder segment.
Mixed salivary gland cells (from passage 9) induced on matrigel differentiated into acini-like structures demonstrating that cells maintained in long-term cultures still have differentiation capacity [ 6, 7].
In addition, IFN-gamma at any passage level induced clearly less I-Ak than was found in C3H 10T1/2 cells, in which I-Ak inducibility was high and stable.
ADE-MSCs from different donors at 0 1 passages were induced to differentiate into adipocytes or osteoblasts for 8 14 days in defined culture conditions.
Our observations of increased DNA damage and CML formation are in line with the well-established fact that increased levels of macromolecular damage, whether spontaneously accumulated during serial passaging or induced by high levels of stress, results in the upregulation of several cell cycle check point regulators [25, 29, 30].
Importantly, disrupting the centrosome in early-passage MEFs induced a loss of centrosome integrity and led to premature senescence, suggesting that centrosomes are involved in MEF senescence.
The third-passage hUCMSCs induced by conditioned medium from developing tooth germ cells (TGC-CM) displayed high alkaline phosphatase (ALP) levels (P < 0.001), an enhanced ability to proliferate (P < 0.05), and the presence of mineralized nodules.
