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While a first passage of an agent between species is not normally sufficient to confirm strain identity, the similarities identified at first passage in this study are striking.
Another study investigating childbearing and kinship in middle class women in the east of India, also concluded that middle class women perceived state-of-the-art medical treatment as part of their rights and privileges [ 12], just as hospital births was seen as a status passage in this study.
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aAlthough Achugar and Schleppegrell (2005) do not refer to the causal process verbs in their study as Causal Material Processes (CMPs), all of the verbs used in the more explicitly causal passages in this study realized material processes (e.g., forced and make).
These results were repeated for all passages in this study.
The analysed PbMut lines, however, shared a large proportion of variants, suggesting that the diverse population was not constructed after long-term passages in this study.
Interestingly, in those with 3 mm passages in this study, the union at host-bone-implant interface was observed, with bone formation occurring throughout the scaffolds by eight weeks after implantation but not in those without a passage.
Matsumoto et al. used ASCs and DFAT cells at passage 1 [ 10], whereas both cell types were used at passage 2 in this study.
According to these data, we can prepare a sufficient amount of human synovial MSCs at passage 0. In this study, we did not use scaffolds for transplantation of aggregates of synovial MSCs.
Cultures of P0 P3 were photographed when they were almost semiconfluent, denoting that cellular morphology and proliferative potential were maintained in the passages adopted in this study.
Early passage HDF used in this study were at passage 15 and MTF at passage 3. HDF were cultured until proliferation ceased (monitored by daily microscopic observation) and incubated for 2 more weeks prior to harvest.
However, it should be noted that this was observed in HCAECs at passage 6~7 (used in this study) but not at passage 2, although there was no obvious difference in morphology under microscope observation up to passage 10 (data not shown).
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