Therefore, using these two set ups allows us to explore if co-occlusion, presumably occurring after the first passage in the serial-passage experiment, has any effect on the persistence of the genotypes.
Parallel, serial passage experiments (PSPEs) have been previously described for Yersinia pestis [ 25] and for Escherichia coli O157 H7 [ 20].
The experiments have been carried out up to gas flows of 5000 ml/min STP per passage.
Serial passage experiments in agriculture have shown that there can be tradeoffs in the ability to infect multiple hosts, where an increase in virulence on a new host is accompanied by a loss of virulence on the original host (Ao and Griffiths 1976; Harrower 1977; Bardin and Loeonard 2000).
Cells used in the experiments had a history of 20 40 passages from the stage of primary culture.
Still, reduced lag time in the sort of serial passage experiment we performed has already been suggested for other bacteria [15], [16].
Previous serial passage experiments with pathogenic fungi and oomycetes have focused primarily on changes in damage to the host, and seldom measure changes in the ability of the pathogen to infect the host per se.
Somehow, this sense of daring experiment had been smothered in memory by the opening pastoral passages and the basic sensuality of the whole.
Through single- and serial-passage experiments, we show that vEGTDEL has lower within-host fitness relative to the parent Wt L1 genotype.
After the third passage, cells have been used for subsequent in vitro and in vivo experiments.
