Tumorigenicity increased significantly on repeated animal passage but oestrogen dependence was retained.
These ER-positive tumors retain their hormone receptor status over multiple passages and their dependence on estrogen for growth.
The resulting mean first passage time (MFPT) has 'U' curve dependence on the temperature.
The well-known kinetic chevron rollover behavior is recovered from the mean first passage time, with the U-shape dependence on temperature.
A strong dependence of the final salt passage on ionic strength and temperature of the annealing solution is demonstrated.
The remaining NS/PC cultures were propagated either as floating neurospheres or as adherent monolayers with mainteinance of growth factor dependence and multipotentiality at late passage.
Disadvantages are the dependence of gene expression on passage number, genomic instability, leading to dedifferentiated cells whose phenotype no longer resembles that of the cell in vivo.
As noted above, a striking difference is the distance-dependence of the mean first passage time (MFPT) of the searcher to the target site.
T-IhNSCs' growth rate was stable over passaging and so was their strict growth factor dependence.
Low dependence on glutamine was confirmed by passaging cells in glutamine-free medium and agrees well with the observation that ammonia is accumulating to very low concentrations in the medium.
