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At second passage, the mean incubation period for the BSE agent appeared to be shortened but, unexpectedly, the incubation period for the FSE agent appeared to be longer compared to first passage data; this was not statistically significant, however.
However TGFβ2 had no significant effect on the fold expansion and the rate of LTP cancer cells self-renewing symmetric divisions based on modeling of serial passage data (Fig. 5b c).
Notably, the five contingency loci with altered expression after three passages in mice also showed significant changes by microarray in C. jejuni isolated after a single passage (data not shown).
Although a number of male chimeras with significant coat colour contribution were generated, a poor rate of germ line transmission was observed even at early passage (data not shown) corresponding to the original findings of Auerbach et. al. [10] with their B6-derived ES cells.
On the other hand, the BHK cells infected with strain 007Lm were revealed to be transferred up to the 6th passage (data not shown).
However, when IMR-32 cells were cotreated with SB203580 and etoposide, the formation of NBSs was totally prevented, even from the first passage (data not shown).
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The described integral equation estimation method also provides a subjective graphical test of the applicability of the OU process or the Feller process when applied to a reasonably large sample of observed first-passage data.
Continued culture in this manner resulted in the progressive loss of mCherry expression over several passages (data not shown).
They could be further expanded in culture for several passages (data not shown); showed significant proliferation as indicated by Ki-67 staining (Fig. 3D); and co-expressed endogenous Nestin (Fig. 3E).
This time point was chosen because maximal VLP formation was also found to be time dependent and optimal at 48 hours post-passage (data not shown).
This increased level of CD133 expression remained stable for up to 72 passages (data not shown).
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