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To determine if the maintenance and expression of full complements of lux genes was detrimental to cellular growth rates in HEK293 cells, the rates of growth among wild-type, pLuxCDEfrp CO, and pLuxCDEfrp:WT containing cells was monitored over the course of a normal passage cycle.
For treatments, cells were cultured using the same method as untreated cells for one passage cycle.
In addition, we extended the database schema of BioSQL to include virus morphology description, geographical information, clinical characteristics, isolation location and year, culture passage cycle, and controlled vocabularies.
The passage cycle was repeated by harvesting plaques, removing the bacteria by filtration, and mixing viruses and naïve (non-coevolving) bacteria at the controlled MOI.
Bacterial evolution was inferred from comparison of resistance between naive t0 bacteria and t1 bacteria, exposed to phage during the first passage cycle.
Phages were confronted with the naive t0 bacteria and with t1 bacteria, with which they had interacted during the first passage cycle (total number of replicates in assay: 4 phage isolates × 8 replicates × 2 bacteria types = 64).
Similar(53)
A total of 60 passage cycles were conducted [ 10].
Although the album was built around "Wave," the keystone is set at the beginning, with a string passage called "Cycle," less than a minute long, which recurs later under various songs.
Hearteningly, traffic laws and roadways saw signs of adaptation, with cities such as Copenhagen installing traffic lights intended to make passage by cycle easier and many other urban centres pushing for lowered speed limits.
The passage 3 growth cycle of BMSCs from normal and GIOP rats was monitored by using flow cytometry.
Phosphorylation of pocket proteins via activated cyclin CDK complexes results in release of E2F transcription factors, allowing for passage through cell cycle checkpoints (Beijersbergen et al., 1995; Dimova and Dyson, 2005).
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