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In each passage, cells were infected with the passaged viruses (∼MOI of 0.1).
Third to fifth passage cells were used for next experiments.
Cells were cultured for a maximum of 4 weeks before thawing fresh, early passage cells.
Early passage cells demonstrated increased proliferation with flow compared to static conditions (36% increase, day 5, P = 0.03); late passage cells had no statistically significant increase (P = 0.42).
PCNA staining confirmed lack of proliferation in late passage cells under static or flow conditions.
(4) Only early passage cells were used to maintain a stable cell phenotype.
Similar(6)
A large of reports about MSC deal with early-passage cells.
Second- or third-passage cells were used for all experiments.
All experiments were conducted using first-passage cells.
All experiments were done on low-passage cells.
Only first-passage cells were used in our experiments.
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