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We have also examined the role of divalent metal ions in strand passage activity and in DNA binding.
C-terminally truncated enzymes had similar strand passage activity to full length enzymes, but the presence of the opposite C-terminal domain had a large effect, with the topoIIα-CTD increasing activity, and the topoIIβ-CTD decreasing activity.
In vitro data suggest that, while the lack of any C-terminal domain has little effect on activity, the presence of either the topoIIα or β C-terminal domain can affect strand passage activity.
While the absence of the C-terminal domain for topoIIα or β had no effect on strand passage activity, the presence of the C-terminal domain from the opposite isoform had a noticeable effect with a clear trend emerging.
The presence of the β-tail on the topoIIα isoform core gave a statistically significant decrease in strand passage activity compared to the native topoIIα protein, and the presence of the α-tail on the topoIIβ isoform core gave an increase in activity compared to the native topoIIβ protein, although this time not significant, towards that of the native topoIIα protein.
Nest predators affected burrowing behaviour and passage activity at burrow entrances as well as physiological stress responses.
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To monitor the presence of pitted animals (shrews and voles) at burrow entrances, we inserted circular antennas (type EUR 3120, diameter: 5 cm, EURO I.D., Weilerswist, Germany) into each tunnel entrance (circa 5 cm deep) and measured passage activities for 3 d.
In this sense, large individual size [ 20], swimming speed and high energy reserves could facilitate the success of passage, whereas activity, exploratory behavior and sensibility to environmental cues [ 21] could increase the probability to find the fishway entrance.
In our model, we see a slight increase of the activity passage after passage.
Was this passage "analyzing an activity" or "challenging an assumption"?
The passage of electrical activity is assumed to be impeded in satiated areas of the brain.
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