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4. Passage 1 differs from Passage 2 in that only Passage 1 a. provides evidence of linguistic similarities betweentwo languages.
Skin cells from six consented patients were culture-expanded to passage 1.
The 3243A>G heteroplasmy levels were assessed in MiPSC lines every 5 passages from passage 1 to passage 30.
The passage 1 cultures were further used in the cell attachment assay and in a 3-day cell growth experiment.
We designated this point as passage 1.
Attached cells then constituted passage 1.
These cells were considered as being passage 1 MEFs.
Passage 1 cells were used in the present study.
At 72 hours post-infection, virus-containing supernatants (passage 1) were collected.
Cell culture experiments were carried out on passage 1 or 2 MECs.
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MEFs were utilized at passage 1 2.
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