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Following a 5-min delay, contrast-enhanced first-pass imaging was repeated under resting conditions with a second intravenous injection of 0.1 mmol/kg Gd-DTPA.
Stress/rest dynamic first-pass perfusion imaging was performed.
The time has passed when imaging was used to only provide information on tumour morphology.
Subsequently, dual-bolus first-pass perfusion imaging was performed as previously shown [ 7, 26, 54].
Ratiometric Fura-2 AM (excitation 340 and 380 nm, emission 435 nm long-pass, ratio=340/380) imaging was used to monitor changes in intracellular [Ca2+] upon stimulation [ 38, 40].
QD imaging was done using an EGFP long-pass filter cube (Chroma, C49012) with emission optimized for QDs (either 685/70 or 585/20).
Perfusion imaging was performed in three short-axis sections during the first pass of the contrast bolus and repeated at rest at least 20 min later.
The affected child of MTI-165 passed away at the age of 18 months from apnea, and no imaging was available.
"Her imaging wasn't convincing, but her clinical picture was".
Anatomical imaging was first performed.
First-pass myocardial perfusion imaging is based on the use of gadolinium-based contrast agents (GBCA) as a tracer, and fast T1-weighted gradient recalled echo (GRE) or echoplanar imaging (EPI) hybrid techniques [81].
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