In an optimal-performance muffler, the partition volume should be made as small as possible, and the transmission loss value in the target frequency range should be high enough for flow noise reduction in a duct.
This could be attributed to a variability in the partition volume in the Fluidigm 12.765 digital array, since the relative standard uncertainty of the average partition volume for the 12.765 digital array has previously been estimated at 5%% [ 27].
One of the critical factors in the measurement of copy number concentrations by digital PCR is the partition volume.
More extensive studies to verify the accuracy of the partition volume in dPCR would be required to exclude this possibility.
However, this will be limited by the partition volume uncertainty when it becomes a larger contributor to the uncertainty than the precision of copy number estimates.
For duplex copy number ratio measurements, the contribution from partition volume in the uncertainty estimate is minimized since both assays are conducted in the same ddPCR.
ANOVA demonstrated no significant intracartridge (p = 0.801) or intercartridge (p = 0.053) effect on the partition volume confirming that the observed variation in droplet volume between wells was independent of the cartridge.
Since concentration is derived by dividing the copy number estimate by the assay volume, an additional key factor, partition volume (Vd) (and its associated uncertainty) needs to be considered when measuring DNA concentration using digital PCR.
When the number of reactions in digital PCR is less than one thousand, the estimated copy number will tend to be a larger contributor to the uncertainty than the partition volume.
30, 31, 35 All patients underwent a coronal T1-weighted 3-dimensional gradient echo sequence with effective blood nulling and fat suppression due to selective water excitation (repetition time = 10.3 milliseconds, echo time = 4.0 milliseconds, Flip angle = 15, inversion time = 20 milliseconds, field of view = 350 × 300mm, matrix = 256 × 140, 140 partitions, volume thickness = 120 150mm).
