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Samples were then submitted to two partitioning steps respectively against hexane to remove non polar molecules and against diethyl ether to collect compounds with intermediate hydrophobicity.
Movement of molecules in a multimembrane system can be envisaged as a series of partitioning steps through alternating aqueous and lipid compartments, 2076) as shown in Figure 14.
After three partitioning steps, the fractions (PM, ICM and MF) were diluted in 250 mM mannitol, 10 mM HEPES/KOH, pH 7.5) and pelleted by centrifugation at 100,000 × g for 1 h at 4°C.
The L 1 metric (defined as L 1 (x, y)=Σi ∣ xi− yi∣ for vectors x and y) was selected as the 'distance' function for all partitioning steps of the clustering algorithm.
In contrast to reports using 2PAP with vertebrate tissue sources, we found that the initial PEG/Dextran partitioning steps in the 2PAP technique are not effective in differentially partitioning PM from ER membranes.
The most-significant results include the behavior of the optimum lipophilicity (log P of the compound with the maximum concentration in the concentration−lipophilicity profiles) in dependence on the exposure time and on the number of the partitioning steps, as well as the dependence of the time to maximal response on lipophilicity.
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Each partitioning step improves accessibility of decomposed mold pieces.
The reconfiguration occurs only if the HW/SW partitioning step needs it.
The partitioning step can significantly affect the quality of the solution as well as the computational time of the algorithms.
We assume that the reconfiguration aspects are taken into account during the HW/SW partitioning step (decision of task implementation).
This work takes its outset in a research framework facilitating the HW/SW partitioning step for SMEs.
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