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A bioactivity-guided fractionation procedure was developed to identify the active partition fraction.
The stalk partition fraction of biomass increments was unaffected by row spacing, although the start of stalk growth was delayed with decreasing RS in terms of the threshold biomass required for stalk partitioning to commence.
In this study ethanol partition fraction was eluted with acetonitrile: methanol (70: 30) and seven peaks were observed at 230nm.
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(Combretaceae) and of its aqueous (TFAqF), hydroalcoholic (TFHAF), and hexanic (TFHEXF) partition fractions have been evaluated.
Additionally, the total phenolic and flavonoid content of the extract and partition fractions has been determined.
To obtain the partition fractions of TFEE, it was formerly dissolved in a methanol/distilled water solution (1 : 2) and extracted with ethyl acetate.
To better understand the therapeutic use of S. integrifolium, we analyzed the antinitric oxide activities of the crude ethanol extract and different partition fractions.
Table 1 shows the total phenolic (TPC) and flavonoid (PFC) content of TFEE and its partition fractions, catechin, and butylated hydroxytoluene (BHT).
TFEE and its partition fractions (TFAqF, TFHAF, and TFHEXF) were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC).
Among the partition fractions of the crude ethanol extract, the methanol fraction (ME) has the highest phenolic content, the strongest inhibitory effect against nitric oxide (NO) release, and the most potent cytoprotective activity in LPS-treated RAW264.7 cells.
The results of the study of the DPPH free radical scavenging activity of TFEE, partition fractions of TFEE (TFAqF, TFHAF, and TFHEXF), catechin, and BHT are presented in Table 1.
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