Indeed, SlSDG3, SlSDG9, SlSDG6 and SlSDG5 have their highest expression in fruit at 1 cm and 2 cm, SlSDG2, SlSDG14, SlSDG13, SlSDG4 and SlSDG10 have their peak expression in fruit at the 3 cm stage, while SlSDG1 and SlSDG7 are particularly expressed in fruit at MG up to B10 stages.
SlJMJ12, SlJMJ16, SlJMJ5 and SlJMJ13 are particularly expressed in fruit at B10, thus suggesting a role in later processes of fruit and/or embryo/seed development.
We found that IFN-alpha mRNA was particularly expressed in tubular epithelial cells at the intracellular level but also on the luminal side of the tubules in the brush border in patients with class IV lupus nephritis.
Codons that are translated more rapidly or accurately should be preferentially found in the coding sequences of genes, particularly those expressed at high levels.
TMEM16A is particularly expressed in goblet cells.
NCX1 is particularly expressed in the basal ganglia where it represents a key player in controlling ionic homeostasis at both plasma membrane and mitochondrial levels in the striatum19,25,26.
Although the idea of reconstructing the whole transcriptome is intriguing, a quantitative estimate of the expression levels of each isoform is difficult, particularly for transcripts expressed at low levels and/or when more than a few isoforms exist.
We required a model that would estimate hybridization signals as accurately as possible with a robust estimate of the background, particularly those signals expressed at low levels, and that would provide a measure of uncertainty for each probe-set or transcript.
This also limited our ability to reconstruct full length transcripts, particularly for those expressed at low level.
